By Peter Sass (eds.)
This quantity presents cutting-edge and novel equipment on antibiotic isolation and purification, identity of antimicrobial killing mechanisms, and strategies for the research and detection of microbial variation ideas. Antibiotics: tools and Protocols courses readers via chapters on construction and layout, mode of motion, and reaction and susceptibility. Written within the hugely profitable Methods in Molecular Biology series layout, chapters contain introductions to their respective subject matters, lists of the required fabrics and reagents, step by step, without problems reproducible laboratory protocols, and tips about troubleshooting and heading off identified pitfalls.
Authoritative and state-of-the-art, Antibiotics: tools and Protocols goals to motivate clinical paintings within the interesting box of antibiotic research.
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Additional resources for Antibiotics: Methods and Protocols
Thereby, the “find homologous gene clusters” and “find known homologous gene clusters” views in antiSMASH may be helpful. A comparison of A. orientalis HCCB10007 cluster 3 with the respective MIBiG entry for vancomycin reveals high similarity in the modular structure as well as high similarity in the set of flanking genes and therefore allows estimating the gene cluster boundaries by simple comparison. Although antiSMASH usually overestimates the gene cluster size, sometimes the known cluster is even bigger than the cluster determined by antiSMASH.
ACS Chem Biol 7(11):1918–1928 Matsui T, Yamane J, Mogi N, Yamaguchi H, Takemoto H, Yao M, Tanaka I (2012) Structural reorganization of the bacterial cell-division protein FtsZ from Staphylococcus aureus. Acta Crystallogr D Biol Crystallogr 68(Pt 9):1175–1188 Brötz-Oesterhelt H, Beyer D, Kroll HP, Endermann R, Ladel C, Schroeder W, Hinzen B, Raddatz S, Paulsen H, Henninger K, 83. 84. 85. 86. 87. 88. 89. 90. 91. Bandow JE, Sahl HG, Labischinski H (2005) Dysregulation of bacterial proteolytic machinery by a new class of antibiotics.
Khosla C, Herschlag D, Cane DE, Walsh CT (2014) Assembly line polyketide synthases: mechanistic insights and unsolved problems. Biochemistry 53(18):2875–2883. 1021/ bi500290t 12. Piel J (2010) Biosynthesis of polyketides by trans-AT polyketide synthases. Nat Prod Rep 27(7):996–1047. 1039/b816430b 13. Challis GL, Naismith JH (2004) Structural aspects of non-ribosomal peptide biosynthesis. Curr Opin Struct Biol 14(6):748–756. 005 Genome Mining for Secondary Metabolites 14. Strieker M, Tanovic A, Marahiel MA (2010) Nonribosomal peptide synthetases: structures and dynamics.
Antibiotics: Methods and Protocols by Peter Sass (eds.)